Determination of Selenium in Edible Mushrooms by Ultrasonic Assisted Water Bath Digestion-Atomic Fluorescence Spectrometry

doi:10.7506/spkx1002-6630-201224065

Abstract

Abstract: A method to determine selenium in edible mushroom was developed using ultrasonic assisted water bath digestion followed by atomic fluorescence spectrometry (AFS). The optimum instrumental parameters were established using an orthogonal array design. Besides, the effects of digestion methods and digestion in the presence or absence of potassium ferricyanide on the results of AFS were investigated. AFS was used to determine the selenium contents of different edible mushrooms and the causes of selenium enrichment and the food safety were analyzed based on the results obtained. The limit of detection of the AFS method was 0.20 ng/mL. The sample pretreatment was more efficient and the results of AFS obtained using ultrasonic assisted water bath digestion showed no significant differences compared with wet digestion. AFS can provide a rapid and accurate to analyze selenium in edible mushroom.

Key words: ultrasonic, water bath, atomic fluorescence spectrometry (AFS), edible fungi, selenium

CLC Number:

TS207.3

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