Biological Properties of Staphylococcus aureus Phage and Its Antibacterial Effect in Milk

doi:10.7506/spkx1002-6630-201311033

Abstract

Abstract:

By the combined use of drop method and double-layer plate method, a phage with specific lytic activity against
Staphylococcus aureus was isoalted from urban sewage and named as qdsa001. The host strain used was Staphylococcus
aureus ATCC 6538. Some physiochemical and biological characteristics of the pahge qdsa 001 were identified. Besides, its
antibacterial effect in milk was also investigated. Transmission electron microscopic (TEM) observation showed that qdsa 001
belonged to the Siphoviridae family. The phage had a strong endurance to temperature below 60 ℃, and its optimal pH and
MOI (multiplicity of infection) were approximately 7－8 and 0.1, respectively. The one-step growth curve plot showed that the
latent period and lytic period of this phage were 10 min and 90 min, respectively. In addition, the average burst size was 32.2.
Therefore, phage qdsa 001 is a virulent bacteriophage and can effectively inactivate Staphylococcus aureus in ready-to-eat milk.

Key words: Staphylococcus aureus, bacteriophage, physiological property, biological control

CLC Number:

CAI Tian-shu，WANG Jing-xue*，LIN Hong，KONG Ling-hong，LI Meng. Biological Properties of Staphylococcus aureus Phage and Its Antibacterial Effect in Milk[J]. FOOD SCIENCE, 2013, 34(11): 147-151.

References

[1] Pesavento, G., Ducci, B., Comodo, N., Nostro, A.L.. Antimicrobial resistance pro?le of Staphylococcus aureus isolated from raw meat: a research for methicillin resistant Staphylococcus aureus (MRSA)[J]. Food Control, 2007, 18(3): 196-200.
[2] Gupta, R., Prasad,Y. P-27/HP Endolysin as Antibacterial Agent for Antibiotic Resistant Staphylococcus aureus of Human Infections[J]. Current microbiology, 2011, 63(1): 39-45.
[3] Franklin D. Lowy, M.D. Staphylococcal aureus infections[J]. N Engl J Med, 1998, 339:520-532.
[4] 刘秀梅. 食源性疾病监控技术的研究[J]. 中国食品卫生杂志, 2004, 16(1): 3-9.
[5] 巢国祥, 焦新安, 周丽萍, 等. 食源性金黄色葡萄球菌流行特征、产肠毒素特性及耐药性研[J]. 中国卫生检验杂志, 2006, 16(8): 904-907.
[6] 黄冰, 邓志爱, 谭铭雄, 等. 食品中金黄色葡萄球菌污染状况、产肠毒素特性及耐药性研究[J]. 中国卫生检验杂志, 2009, 19(6): 1380-1382.
[7] Hagens S, Loessner MJ. Bacteriophage for biocontrol of foodborne pathogens: calculations and considerations[J]. Current Pharmaceutical Biotechnology, 2010, 11(1): 58-68.
[8] 刘伟, 王学琴, 董世雷, 等. 噬菌体及其内溶素的应用研究进展[J]. 浙江农业学报, 2010, 22(5): 702-708.
[9] R M Carlton, W H Noordman, B Biswas, et al. Bacteriophage P100 for control of Listeria monocytogenes in foods: genome sequence, bioinformatic analyses, oral toxicity study, and application[J]. Regulatory Toxicology and Pharmacology, 2005, 43(3): 301-312.
[10] 杜崇涛. 大肠杆菌O157噬菌体的分离鉴定及其初步应用[D]．长春: 吉林大学, 2008．
[11] Lu Z, Breidt FJ, Fleming HP. Isolation and characterization of a Lactobacillus plantarum bacteriophage ΦJL21 from a cucumber fermentation[J]. Food Microbiol, 2003, 84(2): 225-235.
[12] Beatriz Martínez, Jose María Obeso, Ana Rodríguez, et al. Nisin-bacteriophage crossresistance in Staphylococcus aureus[J]. International Journal of Food Microbiology, 2008, 122(3): 253-258.
[13] 洪健, 周雪平. ICTV第八次报告的最新病毒分类系统[J]. 中国病毒学, 2006, 21(1): 84-96.
[14] 于龙, 温占波, 杨文慧, 等. 一株粘质沙雷氏菌烈性噬菌体污水分离及特性[J]. 微生物学报, 2008, 48(4): 498-502.
[15] HENDRIX R W. Bacteriophage genomics[J]. Curr Opin Microbiol, 2003, 6(5): 506-511.
[16] 石琰璟, 郭清吉, 葛晓萍, 等. 金黄色葡萄球菌噬菌体的分离纯化[J]. 青岛科技大学学报, 2007, 28(3): 205-207.
[17] 李陇平, 张智英. 金黄色葡萄球菌烈性噬菌体的分离鉴定和最佳保存方法研究[J]. 中国畜牧兽医, 2011, 38(6): 141-146.
[18] 李波. 原料奶中病原菌的调查及代表性病原菌的研究[J]. 食品工程, 2007, (3): 3-6.