Mathematical Modeling of Glutathione Production by Saccharomyces cerevisiae in Batch Fermentation at Different pH

doi:10.7506/spkx1002-6630-201313035

Abstract

Abstract:

A mathematical model of glutathione production by Saccharomyces cerevisiae Y518 in batch fermentation at
different pH was established with malt sprout extract as the fermentation medium. The results showed that Gaussian fitting
model was in good agreement with the experimental observations for glutathione production at different pH, with correlation
coefficient (R2) of 0.99235 (pH5.0) and 0.98842 (pH6.0), but not suitable for biomass and substrate consumption prediction.
Glutathione production reached the maximum of 72 mg/L at 32 h yielding a biomass of 4.59 g/L when pH was kept constant
at 5.0. In contrast, maximum glutathione production of 91 mg/L was also obtained at 32 h along with a biomass of 4.11 g/L
when pH was kept constant at 6.0.

Key words: glutathione, malt sprout extract, Saccharomyces cerevisiae, Gaussian fitting

CLC Number:

TQ920.1

ZHENG Li-xue1,2，XIE Qun-fan1，WANG Li-mei1,2，QI Bin1,*. Mathematical Modeling of Glutathione Production by Saccharomyces cerevisiae in Batch Fermentation at Different pH[J]. FOOD SCIENCE, 2013, 34(13): 162-164.

References

[1] 程元恺. 谷胱甘肽的解毒作用与毒性代谢物[J]. 生物化学与生物物理进展, 1994, 21(5): 395-399

[2] Izawa S, Inoue Y, Kimura A. Oxidative stress response in yeast: effect of glutathione on adaptation to hydrogen peroxide stress in Saccharomyces cerevisiae[J]. FEBS Lett., 1995, 368(1): 73-76

[3] Sies H, Glutathione and its role in cellular functions[J]. Free Radical Biol, Med., 1999, 27: 916-921

[4] LIANG Guobin, LIAO Xianyan, DU Guocheng, et al. Optimization of amino acids addition for efficient production of glutathione in Candida utilis[J]. Biochemical Engineering Journal, 2008, 41(3): 234-240

[5] A. Kademi, J. Baratti. Batch fermentation kinetics of ethanol production by zymomonas mobilis on cellulose hydrolysate[J]. Biotechnology letters, 1996, 18(6): 643-648.

[6] 代志凯, 印遇龙, 阮征. 微生物发酵动力学模型及其参数拟合的软件实现[J]. 计算机与应用化学, 2011, 28(4): 437-441.

[7] 夏露, 朱艳彬, 刘振鸿, 李小维. 甲醇产纤维素菌株的静态发酵动力学研究[J]. 生物技术, 2011, 21(2): 70-73.

[8] 黎继烈, 崔培梧, 李忠海. 柚苷酶产生菌Penicillium sp.1523分批发酵动力学研究[J]. 中国食品学报, 2012, 12(1): 32-37.

[9] 廖明义, 陈雯莉. 桔青霉发酵制备核酸酶P1的发酵动力学研究[J]. 食品工业科技, 2011, 33(3): 180-183.

[10] 赵从凯, 王洪波, 丁雪珍, 郝会军, 陈美霞. 灰树花液体深层发酵动力学模型研究[J]. 潍坊高等职业教育, 2008, 4(1): 54-56.

[11] 郑丽雪, 王立梅, 梅艳珍, 齐斌. 酿酒酵母生产谷胱甘肽分批发酵动力学研究[J].食品科学, 2011, 32(01): 158-161.

[12] 郑丽雪, 胡耀辉, 齐斌. 酿酒酵母生产谷胱甘肽的培养条件的研究[J].食品科学, 2007, 28(10): 391-395.

[13] 张惟杰. 糖复合物生化研究技术[M].杭州：浙江大学出版社，1994:10.

[14] 何俊勇. 3.7L自动发酵罐扩大培养生产谷胱甘肽的初步研究[J].饲料工业,2005,26(10): 53-55.

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