Abstract:

Objective: To establish a method for simultaneous detection of three species of foodborne pathogenic
bacteria using liquid gene chips. Method: Three sets of specific primers and probes were designed according to the
gene 23S rDNA of Staphylococcus aureus, the gene iap of Listeria monocytogenes and the gene ipaH of Shigella
spp. The multiple PCR reaction systems gave rise to target fragments of 246, 112 bp and 174 bp, respectively. The
target fragments were captured by microspheres coupled with the gene-specific probes, finally establishing liquid
gene chips. Results: The method was highly sensitive and specific. The sensitivity for Staphylococcus aureus, Listeria
monocytogenes and Shigella spp. were 38, 44 CFU/mL and 21 CFU/mL, respectively. Conclusion: We here report
a novel detection assay using liquid gene chips for three foodborne pathogenic bacteria, which provides a highthroughput
system for rapid detection of foodborne pathogenic bacteria.

Key words: liquid gene chip, Staphylococcus aureus, Listeria monocytes, Shigella spp.