Abstract:

The effect of leucine on crucial kinases involved in the mTOR signalling pathway in myogenic satellite cells
was investigated. In porcine satellite cells, 2 mmol/L leucine was added, and western blotting was chosen to illuminate
the influence of leucine on the phospharylation of mTOR, S6K, 4EBP1, TSC2 and Akt. Meanwhile, protein synthesis and
DNA amplification were conducted. Results indicated that the phospharylation of mTOR, S6K, 4EBP1, TSC2 and Akt was
significantly increased. When rapamycin as an inhibitor of mTOR was added in the satellite cells, protein synthesis and
DNA amplification were all terminated in spite of the activation of leucine. In a word, leucine can activate satellite cells by
activating crucial kinases associated with mTOR signaling pathway.

Key words: leucine, satellite cell, mTOR, signaling pathway, kinase