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Optimization of Process Conditions for Chitosan-immobilized SOD from Sheep Blood and Its Enzymatic Characteristics

FU Wen-li1,YANG Xue-shan1,*,YANG Xiao-pu1,ZHU Xia2,HAN Shun-yu2,DU Na2,LI Wei-wei1,BAI Yu1   

  1. 1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
  • Online:2013-10-15 Published:2013-09-27
  • Contact: YANG Xue-shan1

Abstract:

The process conditions for immobilizing SOD from sheep blood by chitosan were explored. Chitosan dosage,
glutaraldehyde concentration, reaction time and pH were optimized by orthogonal array design and a comparative evaluation
of enzymatic properties was made between free and immobilized SOD. The immobilization conditions were optimized as
following: enzyme activity of 5219.3 U/mg, chitosan dosage of 0.25 g, glutaraldehyde concentration of 0.7%, reaction time
of 2 h and pH 5.6. Under the optimal process conditions, the highest activity of immobilized enzyme was obtained, and the
thermal stability was increased by 65.2% when compared with natural SOD. Meanwhile, the resistance to acid and alkali
demonstrated an increase of 48.4%, and Km was 0.18 mmol/L. Chitosan is useful to prepare high-performance immobilized
SOD from sheep blood.

Key words: sheep blood SOD, chitosan, immobilization, enzyme activity

CLC Number: