Abstract:

On the basis of one-factor-at-a-time experiments, Box-Behnken design (BBD) and response surface methodology
(RSM) were applied to explore the effects of acetic acid concentration, material/liquid ratio and extraction duration on the
extraction efficiency of collagen from common carp fish scale. As a result, a mathematical model was fitted. The optimal extraction
conditions were found to be 0.54 mol/L acetic acid as the extraction solvent with a material/liquid ratio of 1:10 (g/mL) for an
extraction duration of 42 h. Under these conditions, the extraction efficiency of collagen was 15.89%.

Key words: Yellow River common carp scale, collagen, response surface methodology, extraction process, optimization