Abstract:

In acetic acid/sodium acetate buffer solution, based on the obvious quenching effect of nickel (Ⅱ) on the
fluorescent intensity of norfloxacin, a fluorescent spectroscopic method for the determination of trace nickel was developed.
Under the optimal experimental conditions, the fluorescent quenching strength responded linearly with nickel (Ⅱ)
concentration in 5.00 × 10-7 to 1.00 × 10-5 mol/L with a detection limit of 2.1 × 10-7 mol/L. The relative standard deviation
was 1.5% (c = 4. 00 × 10-6 mol/L，n = 11). This method has been successfully applied to the determination of nickel (Ⅱ) in
simulated waste water and celery samples, with recovery between 99.60% and 108.8%.

Key words: nickel (Ⅱ), norfloxacin, fluorescence quenching, simulated wastewater, celery