FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (22): 59-63.doi: 10.7506/spkx1002-6630-201322012

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Optimization of Pigment Extraction from Lagerstroemia indica Flowers by Response Surface Methodology

YAN Li,WANG Li-zhi,MA Ming-you   

  1. 1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China;
    2. College of Resource and Planning Sciences, Jishou University, Zhangjiajie 427000, China;
    3. Experimental Equipments Administration Center, Jishou University, Jishou 416000, China
  • Received:2013-01-19 Revised:2013-10-13 Online:2013-11-25 Published:2013-12-05

Abstract:

A three-variable, three-level Box-Behnken experimental design combined with response surface analysis was
employed to optimize the conditions for pigment extraction from Lagerstroemia indica flowers. The extracted pigment was
determined by measuring the absorbance at the maximum absorption wavelength of 546 nm. The best solvent for the extraction
of pigment was found to be 50% ethanol. The optimal conditions for ultrasonic-assisted extraction were determined as
400 W ultrasonic power, 22 min irradiation time and a liquid-material ratio of 45:1 (mL/g). The predicted absorbance of pigment
extracts under the optimized conditions was 0.608 and the actual value was 0.610, showing a small error between both.
Thus, the optimized extraction conditions are reliable.

Key words: Lagerstroemia indica flowers, pigment, response surface methodology, extraction

CLC Number: