Rapid and High Throughput Quantitative Analysis of Catechin Polyphenols in Tea Beverages by Ultra-High Performance Liquid Chromatography with Photodiode Array Detection (UPLC-PDA)

doi:10.7506/spkx1002-6630-201322034

Abstract

Abstract:

An efficient and high throughput analytical method was developed for the determination of galiic acid (GA) and
five catechin polyphenols including catechin (C), epicatechin (EC), epigallocatechin gallate (EGCG), epicatechin gollate
(ECG), and epigallocatechin (EGC) in tea. A Kinetex-C18 (2.1 mm×100 mm, 1.7 μm) column was employed at 45 ℃.
Gradient elution was applied with 0.1% acidic methane (V/V) and acetonitrile (from 5% to 30% in 10 min) as the mobile
phase at a flow rate of 0.3 mL/min. The detector wavelength was set at 280 nm. The results demonstrated that six standard
polyphenols could be separated in approximately 7 min. A good linear relationship between polyphenol concentration and
peak area was observed in the range of 0.25－50 mg/L for GA and C and of 1－100 mg/L for EGC, EC, EGCG and ECG,
with correlation coefficients of 0.9993－0.9999. Samples were purified by solid phase extraction. The recoveries of six
polyphenols were in the range of 92.5%－98.5% (GA), 95.9%－98.5% (ECG), 97.8%－101.0% (C), 95.5%－96.7% (EC),
95.5%－98.9% (EGC), and 95.1%－103.4% (EGCG), with relative standard deviation (RSD) of 0.8%－4.7%, respectively.

Key words: ultra performance liquid chromatography (UPLC), photodiode array detector (PDA), quantification, catechin polyphenols, tea beverages

CLC Number:

O656.21

ZHANG Shuang，HUANG Meng-tian，JIAO Yan-jin，TAO Guan-jun，ZENG Mao-mao，CHEN Jie. Rapid and High Throughput Quantitative Analysis of Catechin Polyphenols in Tea Beverages by Ultra-High Performance Liquid Chromatography with Photodiode Array Detection (UPLC-PDA)[J]. FOOD SCIENCE, 2013, 34(22): 170-173.

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