Effects of A Glycoprotein from Jellyfish (Rhopilema esculentum) on Cytokine mRNA Expression in BALB/c Mice

doi:10.7506/spkx1002-6630-201323061

Abstract

Abstract:

In a previous study, we isolated a glycoprotein named JGP-III from jellyfish (Rhopilema esculentum). The specific
aim of the present study was to investigate the roles of JGP-III in the immunoregulatory mechanism in BALB/c mice by
investigating its effects on the mRNA expression of the Th1- and Th2-cytokines in the spleen lymphocytes of these mice. A total of
54 healthy BALB/c mice (SPF, 18－22 g) were randomly divided into 6 groups with 9 mice in each group: normal control, model
control, positive control, low dose (10 mg/(kg·d)), moderate dose (50 mg/(kg·d)) and high dose (100 mg/(kg·d)) groups, respectively.
After 21 days of these treatments, lymphocytes were prepared from the spleen of these mice and total RNA was
isolated from their lymphocytes. Fluorescent quantitative real-time polymerase chain reaction (FQ-RT-PCR) technique was
used to analyze the relative mRNA expression of Th1-cytokines (IL-2, IL-12 and IFN-γ) and Th2-cytokines (IL-4, IL-6 and
IL-10). Our results showed that JGP-Ⅲ promoted the mRNA expression levels of Th1-cytokines but inhibited the mRNA
expression levels of Th2-cytokines. Moreover, our results suggest that JGP-Ⅲ can improve the body’s immune function
probably by regulating the mRNA expression levels of Th1-/Th2-cytokines in spleen lymphocytes.

Key words: jellyfish, glycoprotein, cytokine, mRNA expression

CLC Number:

TS254.9

REN Guo-yan，SHAO Zheng，CAO Li，CAO Li，ZHANG Fan，GUO Jin-ying，FAN Jin-ling，CHEN Xiu-jin， ZHAO Rui-hong. Effects of A Glycoprotein from Jellyfish (Rhopilema esculentum) on Cytokine mRNA Expression in BALB/c Mice[J]. FOOD SCIENCE, 2013, 34(23): 305-308.

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