FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (13): 216-221.doi: 10.7506/spkx1002-6630-201813032

• Nutrition & Hygiene • Previous Articles     Next Articles

Effects of Pseudobulbus cremastrae seu pleiones Polysaccharide on the Regulation of Immune Function and the Inhibition of Tumor Growth in Sarcoma S180 Tumor-Bearing Mice

JIANG Shuang1, XU Jingyao2, SU Xin1, LIU Zhi1, SONG Wu1,*   

  1. 1. College of Basic Medical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China; 2. Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130021, China
  • Online:2018-07-15 Published:2018-07-09

Abstract: Objective: To explore the effects of Pseudobulbus cremastrae seu pleiones polysaccharide (PCSPP) on the regulation of immune functionand the inhibition of tumor growth in sarcoma S180 tumor-bearing mice. Methods: Lymphocyte proliferation capacity and the phagocytic activity of spleen macrophages were determined by 3-(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and neutral red methods; the effects of PCSPP on tumor growth, spleen index and thymus index in S180 tumor-bearing mice were evaluated through tumor suppression test in vivo, and the effects on T lymphocyte subgroup and serum cytokine levels were detected by flow cytometry and enzymelinked immunosorbent assay, respectively. Results: PCSPP could enhance lymphocyte proliferation capacity and macrophage phagocytic activity. PCSPP could inhibit tumor growth and increase spleen index and thymus index in S180 tumor-bearing mice; meanwhile, it could also increase the levels of CD4+ and CD8+ T cells in mice and elevate the CD4+/CD8+ ratio, interleukin-2, tumor necrosis factor-α and interferon-γ levels. Conclusion: PCSPP has a potent antitumor effect and its inhibitory effect may be related to ithe improvement of immune function.

Key words: Pseudobulbus cremastrae seu pleiones polysaccharide, sarcoma, immunoregulation, cytokines

CLC Number: