Preparative Isolation and Purification of Corosolic Acid from Schisandra chinensis (Turcz.) Baill by High Speed Counter Current Chromatography

doi:10.7506/spkx1002-6630-201402001

Abstract

Abstract:

An effective and rapid method for the separation of corosolic acid from Schizandra chinensis (Turcz.) Baill by high-speed counter-current chromatography (HSCCC) was established．The alcohol extract of Schizandra chinensis (Turcz.) Baill was initially separated using AB-8 macroporous resins and further purified by HSCCC．The high performance liquid chromatography ( HPLC) ) method was used to determine the partitioning coefficient of crude extract in different solvent systems．The results showed the solvent system of n-hexane-ethyl acetate-methanol-water (6:5:5:4, V/V/V/V) was the best one for the HSCCC separation．A total of 6.3 mg of corosolic acid with a high purity of 97.6% was obtained in one step separation from 150 mg of crude extract. The method is of high separation efficiency, and great importance for the applications of corosolic acid in the food and medical fields.

Key words: high-speed counter-current chromatography, corosolic acid, Schisandra chinensis (Turcz.) Baill

CLC Number:

TS201.3

ZHU Li-jie, MENG Xian-jun*, LI Bin, LIU Xiu-ying, TAO Dong-bing. Preparative Isolation and Purification of Corosolic Acid from Schisandra chinensis (Turcz.) Baill by High Speed Counter Current Chromatography[J]. FOOD SCIENCE, 2014, 35(2): 1-4.

References

[1] Hancke J L, Burgos R A and Ahumada F. Schisandra chinensis (Turcz.) Baill[J]. Fitoterapia, 1999, 70: 451-471.

[2] 戴好富, 谭宁华, 周俊. 北五味子中的一个新单萜苷[J]. 高等学校化学学报, 2005, 26(9): 1659-1661.

[3] 史琳, 王志成, 冯叙桥. 五味子化学成分及药理作用的研究进展[J]. 药物评价研究, 2011, 34(3): 208-212.

[4] 黄晓东, 任旷. 五味子木脂素研究进展[J]. 吉林医药学院学报, 2010, 31(1): 46-49.

[5] 游玟娟, 温拥军. 北五味子多糖提取条件及其抗氧化性能[J]. 食品与发酵工业, 2012, 38(9): 206-210.

[6] 唐苗苗, 刘翼翔, 毛婷等. 高效液相色谱法测定北五味子有机酸含量[J]. 食品工业科技, 2012, 33(15): 328-330.

[7] 梁茂雨, 陈怡平, 纵伟. 降血糖功能因子科罗索酸研究进展[J]. 中国食物与营养, 2007, 5:17-18.

[8] 欧阳慧, 封亮, 徐友华等. 不同产地夏枯草中抗非小细胞肺癌成分科罗索酸的差异比较[J]. 中华中医药杂志, 2012, 27(6): 1571-1574.

[9] Kyoung J N, Jin M C and HoKyoung K. Corosolic acid induces apoptotic cell death in human lung adenocarcinoma A549 cells in vitro[J]. Food and Chemical Toxicology, 2013, 56: 8-17.

[10] 沈驹华, 何洁, 韦盛. 科罗索酸对大鼠嗜铬细胞瘤PC12细胞缺氧缺糖损伤的保护作用[J]. 中国临床药理学杂志, 2010, 26(12): 932-935.

[11] 王冠. 瑶药白马骨化学成分及生物活性初步研究[D]. 广州: 暨南大学, 2006.

[12] Oka H, Harada K I and Ito Y. Separation of antibiotics by countercurrent chromatography[J]. Journal of Chromatography A, 1998, 812: 35-52.

[13] 程杰, 符晓晖, 王维娜. 高速逆流色谱在中药分离中溶剂体系的筛选[J]. 中草药, 2008, 39(8): 1272-1275.

[14] 崔恒薇, 李博, 杜琪珍. 高速逆流色谱分离制备常春油麻藤豆荚中丁香脂素[J]. 食品与发酵工业, 2011, 37(5): 186-188.

[15] 钱登勇, 李博, 蒋志国等. 高速逆流色谱分离制备胡椒中的胡椒碱[J]. 食品科学, 2011, 32(24): 274-276.

[16] 蒋志国, 陈文学, 刘四新. 高速逆流色谱分离制备白胡椒中单萜类化合物[J]. 食品与发酵工业, 2011, 37(3): 202-205.

[17] 高荫榆, 魏强, 范青生等. 高速逆流色谱分离提取天然产物技术研究进展[J]. 食品科学, 2008, 29(2): 461-465.

[18] Frighetto T S, Welendorf R M, Nigro E N, et al. Isolation of ursolic acid from apple peels by high speed counter-current chromatography[J]. Food Chemistry, 2008, 106: 767-771.

[19] Yang Y, Gu D, Wu H, et al. Application of preparative high-speed countercurrent chromatography for separation of elatine from Delphinium shawurense[J]. Journal of Liquid Chromatography & Related Technologies, 2008, 31: 3012-3019.

[20] Yao S, Luo J, Huang X, et al. Application of preparative high-speed counter-current chromatography/preparative high-performance liquid chromatography mode in rapid separation of saponins[J]. Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences, 2008, 864: 69-77.

[21] 孟宪军, 朱力杰, 李斌等. 大孔树脂纯化北五味子藤茎中总三萜的研究[J]. 食品工业科技, 2012, 33(4): 339-342.

[22] Zong W and Zhao G. Corosolic acid isolation from the leaves of Eriobotrta japonica showing the effects on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes[J]. Asia Pacific Journal of Clinical Nutrition, 2007, 16: 346-352.