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Purification and Analysis of Proanthocyanidins from Red Peel of Lotus Seeds

LI Qi-li, PENG Fang-Gang, LIU De-ming, LIU Zhen-bao, WU Wei-guo   

  1. 1. Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences,Changsha 410125, China; 3. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Analysis and Testing Center of National Key Laboratory, Hunan Agricultural University, Changsha 410128, China;4. School of Pharmaceutical Sciences, Central South University, Changsha 410013, China
  • Online:2014-02-13 Published:2014-03-17

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Abstract:

Proanthocyanidins, a large class of polyphenols, are widely present in natural plants. In this study,
proanthocyanidins extracted from the red peel of lotus seeds were purified by a macroporous resin AB-8 and a polyamide
column. Chromatographic eluates were analyzed by infrared spectroscopy (IR) and electrospray ionization-mass
spectroscopy (ESI-MS). The separated components of proanthocyanidins were further confirmed by reverse phase highperformance
liquid chromatography-mass spectroscopy (RP-HPLC-MS/MS) to be nine monomers and oligomers of
proanthocyandins including (+)-catechin, (-)-epicatechin (m/z 289), (+)-gallocatechin (m/z 305), four isomeric compounds of
procyanidin dimers (m/z 577) and two tetramers (m/z 1 153).

Key words: lotus seed, proanthocyanidins, infrared spectroscopy, reverse phase highperformance liquid chromatography-mass spectroscopy