Rapid Determination of Melamine and Dicyandiamide in Milk Powder by High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201406037

Abstract

Abstract:

A high performance liquid chromatography-diode array detector (DAD) method was developed for rapid
and simultaneous determination of melamine and dicyandiamide in milk powder. Melamine and dicyandiamide were
extracted with 1% trichloroacetic acid solution. The chromatographic separation was performed on an amino column using
acetonitrile-water (75:25, V/V) as mobile phase at a flow rate of 1.0 mL/min, and the analytes were detected with a DAD
detector at 233 nm. The two compounds were well separated with a good linear relationship in the wide range of 0.005–60 mg/L.
The average recoveries in milk powder were in the range of 83.2%–102.6% at several spiked levels. The lowest limits of
quantification for melamine and dicyandiamide were 0.17 and 0.15 mg/kg, respectively, and the precision of the method
expressed as coefficient of variance (CV) was in the range of 2.3%–7.2% (n = 6). This method is simple, rapid, low-cost and
suitable for the simultaneous determination of melamine and dicyandiamide in milk powder.

Key words: high performance liquid chromatography, diode array detector, milk powder, melamine, dicyandiamide

CLC Number:

TS207.3

YANG Jia1, DING Juan-fang2, ZHOU Yuan-yuan1, XU Qiu1, ZHU Jiang1, WANG Shuai1. Rapid Determination of Melamine and Dicyandiamide in Milk Powder by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2014, 35(6): 172-175.

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