Determination of Phenolic Compounds in Proso Millet by High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201408030

FOOD SCIENCE

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Determination of Phenolic Compounds in Proso Millet by High Performance Liquid Chromatography

LU Rong1, TIAN Qin1, SUN Nan1, QIAO Zhi-jun2, ZHANG Li-zhen1,*, NIU Wei3, SHAN Lu1, JI Pei-shuang1

1. College of Life Science, Shanxi University, Taiyuan 030006, China; 2. Key Laboratory of Crop Gene Resources and Germplasm
Enhancement on Loess Plateau, Ministry of Agriculture, Institute of Germplasm Resources, Shanxi Academy of Agricultural Sciences,
Taiyuan 030006, China; 3. Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China

Online:2014-04-25 Published:2014-05-13
Contact: ZHANG Li-zhen

Abstract

Abstract:

The Folin-Ciocalteu method was used to assay the free phenolic content of proso millet. High performance liquid
chromatography (HPLC) was used to analyze its phenolic composition. The results showed that free phenolic content was not
significantly different among different varieties of millet bran, but significantly different (p ＜ 0.01) in the debranned grain. Free
phenolic content was significantly different (p ＜ 0.05) between the bran and grain from the same variety, and the bran had much
higher free phenolic content. The highest free phenolic content was found in both bran and grain from proso millet Chimi 2, with
159.22 mg gallic acid equiv/100 g bran, and 47.98 mg gallic acid equiv/100 g grain. Chlorogenic acid, syringic acid, vanillic acid,
p-coumaric acid and ferulic acid were the major phenolic acids in proso millet.

Key words: bran, proso millet, phenolic compound, high performance liquid chromatography

CLC Number:

S515

LU Rong1, TIAN Qin1, SUN Nan1, QIAO Zhi-jun2, ZHANG Li-zhen1,*, NIU Wei3, SHAN Lu1, JI Pei-shuang1. Determination of Phenolic Compounds in Proso Millet by High Performance Liquid Chromatography[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201408030.

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Determination of Phenolic Compounds in Proso Millet by High Performance Liquid Chromatography

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