FOOD SCIENCE

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Optimization of Fermentation Conditions for Production of Neutral Protease by Bacillus subtilis 10075

LIU Ying, ZHANG Bin-bin, SUN Bing-yu, LIU Lin-lin, ZOU Li-hong, SHI Yan-guo   

  1. Key Laboratory for Food Science and Engineering of Heilongjiang Province, College of Food Engineering,Harbin University of Commerce, Harbin 150076, China
  • Online:2014-07-15 Published:2014-07-18

Abstract:

The medium components and culture conditions for Bacillus subtilis 10075 were optimized by single-factor
and orthogonal array design methods to increase the production of neutral protease. The results showed that the optimized
medium consisted of 8.0% maltose, 4.0% peptone and 0.08% MgSO4, and the optimized culture conditions were 37 ℃, 7.0
and 42 h for temperature, initial pH and time, respectively. The activity of neutral protease after optimization was increased
from 98.36 to 353.45 U/mL.

Key words: Bacillus subtilis, neutral protease activity, fermentation, optimization