Optimization of High Cell Density Culture Conditions for Microencapsulated Lactobacillus bulgaricus by Response Surface Methodology

doi:10.7506/spkx1002-6630-201417030

Abstract

Abstract:

In this study, the medium composition and culture conditions for microencapsulated Lactobacillus bulgaricus
FMG-4 were optimized using response surface methodology. The results showed that the optimal medium for high cell
density culture of microencapsulated Lactobacillus bulgaricus FMG-4 was an MRS medium supplemented with 97.15 g/L
whey powder, 10.00 g/L soy protein powder, 7.55 g/L yeast powder, 8.03 g/L calcium carbonate, 0.30 g/L magnesium sulfate
and 0.02 g/L manganese sulfate. The optimal culture conditions were 41.7 ℃ and an initial pH of 6.9. Under the optimized
culture conditions, the density of L. bulgaricus FMG-4 reached 3.18×1011 CFU/g.

Key words: liquid core microcapsule, response surface methodology, Lactobacillus bulgaricus, high cell density culture

CLC Number:

TS201.3

YAN Ying-juan, LU Jian, ZHOU Jian-zhong, LI Wei, DONG Ming-sheng. Optimization of High Cell Density Culture Conditions for Microencapsulated Lactobacillus bulgaricus by Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201417030.

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Optimization of High Cell Density Culture Conditions for Microencapsulated Lactobacillus bulgaricus by Response Surface Methodology

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