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Optimization of Extraction of Antibacterial Compounds from Red Ants with Response Surface Methodology

CHEN Hai-yan1, FENG Yin1, YANG Liu1, YOU Li-xin1, CHEN Xiao-ping2,*   

  1. 1. College of Biotechnology and Food Science, Changchun University of Science and Technology, Changchun 130600, China;
    2. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Online:2014-11-25 Published:2014-12-09

Abstract:

Antibacterial compounds were extracted from wild red ants from the Changbai Mountains and their antibacterial
activity was evaluated. The anti-E. coli activity as a function of ethanol concentration, solid-to-solvent ratio and centrifugal
speed was investigated using response surface methodology. The diameter of the inhibition zone against E. coli was 2.59 cm
under the optimal extraction conditions: 63% aqueous ethanol, a solid-to-solvent ratio of 1:26 (g/mL) and centrifugation at a
speed of 7 230 r/min.

Key words: response surface methodology, red ant, antimicrobial compounds

CLC Number: