FOOD SCIENCE

• Analysis & Detection • Previous Articles     Next Articles

Loop-Mediated Isothermal Amplification (LAMP) for Detection of Alicyclobacillus acidoterrestris in Foods

ZHOU Zan-hu1,2, ZHANG Hai-yan3, ZHENG Jun-chao2, JIANG Zhe-min2, CHEN Chun-xiang2, HE Yi-zhen2, LIN Jun-jun2, SU Xue-qin2, WANG Gen-fang2, ZHENG Tian-ling1,*   

  1. 1. Key Laboratory of Coastal and Wetland Ecosystems, Ministry of Education, School of Life Sciences, Xiamen University,
    Xiamen 361005, China; 2. Zhangzhou Entry-Exit Inspection and Quarantine Bureau, Zhangzhou 363000, China;
    3. College of Zhangzhou Health Vocational, Zhangzhou 363000, China
  • Online:2014-11-25 Published:2014-12-09

RichHTML

0

PDF (PC)

266

Abstract:

Purpose: A loop-mediated isothermal amplification (LAMP) method was established for the detection of
Alicyclobacillus acidoterrestris in foods. Methods: After optimization of the reaction conditions of LAMP including the
concentrations of primers, reaction time and amplification temperature, the LAMP method was developed, and its sensitivity
and specificity were evaluated. Results: The method was capable of rapidly and specifically detecting A. acidoterrestris in
foods within 1 hour at a constant temperature of 63 ℃. The sensitivity of the method was 6.7 CFU/mL and the specificity
was 100%. Conclusions: The LAMP method is efficient, highly sensitive and specific, and suitable for the rapid detection of
A. acidoterrestris in various food samples.

Key words: loop-mediated isothermal amplification (LAMP), Alicyclobacillus acidoterrestris, food

CLC Number: