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Screening of Lactic Acid Bacteria with High Yield of Gamma-Aminobutyric Acid from Yellow Serofluid,the Wastewater from Tofu Production

LIU Jia-rong, LIANG Jin-zhong   

  1. Key Laboratory of Food Science and Engineering, School of Food Engineering, Harbin University of Commerce,Harbin 150076, China
  • Published:2014-12-15

Abstract:

After primary screening and rescreening, a high-yield strain producing γ-aminobutyric acid (GABA) was isolated
from yellow serofluid, the wastewater from tofu production. Morphology analysis, physiological and biochemical properties
and 16S rDNA sequences in GenBank indicated that the strain belonged to the Lactobacillus genus. Then, the phylogenic
tree generated by MEGA 6.0 software revealed that it was closely related to Lactobacillus plantarum with a similarity of
99%, and numbered LP-Dfa301. In the fermentation broth, GABA production could reach 5.833 g/L.

Key words: γ-aminobutyric acid (GABA), Lactobacillus, yellow serofluid, 16S rDNA, phylogenic tree

CLC Number: