Abstract:

Response surface design was employed to optimize the extraction of pectin from Jerusalem artichoke pulp.
Different solvents (hydrochloric acid, sulfuric acid, sulphurous acid and phosphoric acid) were compared on the yield of
pectin. Results showed that phosphoric acid revealed better extraction efficiency. Based on single factor experiments, Box-
Behnken Design was used to explore the effects of processing variables (extraction temperature, pH, extraction duration
and liquid-to-solid ratio) on the yield of pectin. Under the optimal extraction conditions: pH 1.52, 63.62 min, 100 ℃ and
a liquid-to-solid ratio of 44.4:1 (mL/g), the maximum predicted pectin yield was 18.76%. Experiments conducted under these
conditions led to an extraction yield of (18.52 ± 0.9)%, which was consistent with the predicted value. Therefore, the established
model was feasible. Moreover, FTIR spectroscopy was used to analyze pectin structure. The obtained product was analyzed for its
physicochemical parameters and all analytical results met the requirements of the national standard (GB 25533—2010).

Key words: Jerusalem artichoke pulp, pectin, response surface methodology, properties