Determination of Relative Molecular Weight Distribution of Polypeptides from Three Different Sources by Gel Filtration Chromatography

doi:10.7506/spkx1002-6630-201424060

Abstract

Abstract:

Objective: To establish a method to determine the relative molecular weight distribution of soy peptide, albumin
polypeptide and sheep placenta polypeptide using gel filtration chromatography (GFC). Methods: TSK-GEL G2000SWXL
(300 mm × 7.8 mm, 5 μm) column was equilibrated with a mobile phase composed of acetonitrile-water-trifluoroacetic acid
(15:85:0.07, V/V) at a flow rate of 0.6 mL/min. The column temperature was 30 ℃ and the injection volume was 10 μL.
The detection wavelength was 220 nm. Results: A linear relationship between the logarithm of relative molecular weight and
retention time was achieved in the relative molecular weight range of 189.1–12 355 with a correlation coefficient of 0.995 6.
The precision, repeatability, stability and accuracy of the GFC method were good. Conclusion: This method is simple, rapid,
sensitive, and reproducible for the determination of the relative molecular weight distribution of polypeptides.

Key words: gel filtration chromatography, soybean, albumin, sheep placenta, polypeptides, relative molecular weight

CLC Number:

O657.7

JIANG Teng-chuan1, SONG Xin-bo1,2,*, ZHANG Li-juan1. Determination of Relative Molecular Weight Distribution of Polypeptides from Three Different Sources by Gel Filtration Chromatography[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201424060.

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Determination of Relative Molecular Weight Distribution of Polypeptides from Three Different Sources by Gel Filtration Chromatography

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