Abstract:

The chemical compositions of short and long chain triglycerides (SLCTs) were analyzed by gas chromatographymass
spectrometry (GC-MS) through mass spectral library searching and characteristic ions. A gas chromatography with
flame ionization detection (GC-FID) method was developed for determining the content of SLCTs in edible oil samples
using DB-17 ht fused silica capillary column as the analytical column. The results showed a good linear correlation between
peak area and concentration in the range of 0.5–20.0 mg/mL. The relative standard deviations for retention time and
peak area of each SLCT were less than 0.05% and 2.0%, respectively, suggesting good reproducibility of the developed
method. The average recoveries from spiked samples of transesterified and original soybean oil were 98.62% and 97.59%,
respectively. This method allowed accurate and highly repeatable quantification of SLCTs in edible oils.

Key words: gas chromatography (GC), short and long chain triglycerides, mass spectrometry (MS), flame ionization detector (FID)