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Isolation and Identification of Lactic Acid Bacteria from Kimchi

MENG Lingshuai1, ZHANG Ying1, ZOU Tingting1, WU Rina1,2, YUE Xiqing1, WU Junrui1,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China; 2. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Online:2015-06-15 Published:2015-06-07

Abstract:

Lactic acid bacteria were isolated and identified from 25 Korean traditional fermented kimchi samples from
7 districts of Shenyang. Totally 81 strains suspected of being lactic acid bacteria were isolated successfully, and 34 of
the isolated strains were identified as bacilli and 47 were identified as cocci. These 81 strains were further identified by
16S rDNA sequence analysis. The results showed that all these 81 strains were confirmed as lactic acid bacteria from 2 genus
and 6 species, including 45 strains of Enterococcus faecium, 25 strains of Lactobacillus plantarum, 4 strains of Lactobacillus
casei, 3 strains of Lactobacillus pentosus, 2 strains of Lactobacillus brevis, and 2 strains of Enterococcus durans. These
results can provide a basis for further studies on lactic acid bacteria from kimchi in northeast China.

Key words: kimchi, Lactobacillus, 16S rDNA, homology analysis

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