Abstract:

We developed a method for the determination of eight naturally occurring sugars in wine by ion chromatography
with integral amperometric detection. We used potassium hydroxide (KOH) produced by eluent generator electrolysis for
gradient elution. On CarboPac PA20 anion exchange column, all eight target compounds could be separated completely in 30
minutes. The limits of detection (LODs) of these analytes by the integral amperometric detector were very low, and a wide
linear range was found. The relative standard deviations (RSDs) for six replicate determinations varied from 4.9% to 8.7%,
and the recoveries of these eight sugars from spiked samples ranged from 81.5% to 95.1%. Our method proved to be simple,
fast, sensitive and suitable for the analysis of the eight naturally occurring sugars in wine by achieving good separation
without derivatization.

Key words: ion chromatography (IC), integral amperometric detector (IAD), wine, naturally occurring sugar