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Development of Enzymatic Electrode for the Detection of Glucose in Not-From-Concentrate (NFC) Fruit Juices

QI Cuihua1, LI Yiwei2,3, ZHANG Xiaowei1   

  1. 1. School of Hotel Management, University of Jinan, Jinan 250022, China; 2. Key Biosensor Laboratory of Shandong Province,
    Biology Institute of Shandong Academy of Sciences, Jinan 250014, China;
    3. School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China
  • Online:2015-07-25 Published:2015-07-15

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Abstract:

A glucose oxidase (GOx) electrode fabricated on the basis of an electron mediator bulk-modified screen-printed
electrode as an electrochemical transducer made from water-insoluble prussian blue (PB), which was easy to prepare, was
reported in this study. Electrochemical characterizations revealed that the enzyme electrode exhibited robust current response
properties. It possessed a linearity toward glucose within the range of 8.0 μmol/L–2.2 mmol/L. Its limit of detection (LOD)
was 0.082 7 μmol/L (RSN = 3), sensitivity was 1.016 μA·L/mmol, response time was 5 s and it showed satisfactory antiinterference
capability, stability and reproducibility. The formulation and working conditions of the electrode were also
optimized. This electrode is easy to prepare, economic and applicable for the rapid assay of glucose in not-from-concentrate
(NFC) fruit juices.

Key words: not-from-concentrate (NFC) fruit juices, glucose, screen-printed electrode, industrial application

CLC Number: