Abstract:

The quercetin content of serum from diet-induced obese mice was determined by high-performance liquid
chromatography fitted with an ultraviolet detector (HPLC-UV) and an Agilent Eclipse XDB-C18 column (4.6 mm × 250 mm,
5 μm). The mobile phase was a mixture of acetonitrile-0.2% formic acid (40:60, V/V) with a flow rate of 0.5 mL/min, and
the detection wavelength was 360 nm. There was a good linear relationship for quercetin in the range of 2.5–40 μg/mL
(r = 0.999 1). The average recovery rate for quercetin was 96.0% with relative standard deviation (RSD) of 10.98% (n = 5).
This method could be used to determine quercetin in serum with good recovery rate and repeatability. It was a simple, rapid
and accurate analytical method.

Key words: high performance liquid chromatography, ultraviolet detector, high fat diet, serum, quercetin