Fast Determination of Iron in Milk Powder by Rayleigh Light Scattering Technique with Bromocresol Green as Probe

doi:10.7506/spkx1002-6630-201520028

Abstract

Abstract:

A new Rayleigh light scattering (RLS) method for quantifying Fe(Ⅲ) was developed. In Tris-HCl buffer medium
at pH 3.4–6.8, Fe(Ⅲ) could be bound to bromocresol green to form a new product, leading to a distinctly enhanced RLS and
the appearance of a new RLS. The maximum Rayleigh scattering peak was located at 339 nm. The RLS intensity (ΔIRLS)
was directly proportional to Fe(Ⅲ) concentration in the range of 0.005 to 0.17 mg/L with the limit of detection being
0.002 4 mg/L. Therefore, a new and accurate Rayleigh light scattering method for quantifying Fe(Ⅲ) was developed. The
optimal reaction conditions and Rayleigh scattering spectral characteristics were investigated. The method has been applied
to the determination of iron in milk powder with satisfactory results.

Key words: bromocresol green, iron, milk powder, Rayleigh scattering

CLC Number:

O657.3

PANG Xiangdong, ZHANG Mei, JIANG Hong. Fast Determination of Iron in Milk Powder by Rayleigh Light Scattering Technique with Bromocresol Green as Probe[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201520028.

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Fast Determination of Iron in Milk Powder by Rayleigh Light Scattering Technique with Bromocresol Green as Probe

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