Abstract:

Polyphenols from Fuzhuan brick tea were obtained through hot water extraction and resin adsorption-separation
process, and their inhibitory effect on pancreatic lipase was investigated by using enzyme kinetic analysis and fluorescence
quenching method. The results showed that the polyphenols from Fuzhuan brick tea had inhibitory activity on pancreatic
lipase with half inhibitory concentration (IC50) of 0.81 mg/mL. The inhibition was a type of reversible non-competitive
inhibition, and the constant of inhibition (Ki) was 2.56 mg/mL. The fluorescence quenching analysis showed that the
polyphenols had quenching effect on pancreatic lipase, and they reduced the enzyme activity, possibly by changing its
structure due to their bonding to pancreatic lipase.

Key words: Fuzhuan brick tea, polyphenol, pancreatic lipase, inhibition activity