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Optimization of Fermentation Conditions for the Production of Phospholipase A2 by Recombinant Kluyveromyces lactis

WANG Hui1,2, ZHANG Liang1,2,*, LI Youran2, GU Zhenghua2, DING Zhongyang2, SHI Guiyang2, CHEN Guoan3, YANG Shengrong3   

  1. 1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;
    2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;
    3. Wuxi Jiangda Baitai Technology Co. Ltd., Wuxi 214122, China
  • Online:2015-11-15 Published:2015-12-03

Abstract:

Fermentation conditions for the production of phospholipase A2 (PLA2) by recombinant Kluyveromyces lactis
GG799 were optimized using single factor and orthogonal design experiments. The optimal fermentation medium was
composed of 30 g/L glucose, 20 g/L yeast extract, 30 g/L peptone and 3 g/L KH2PO4. The best fermentation conditions were
determined as 30 ℃,2%, 7.0, 90 mL and 220 r/min for temperature, inoculum amount, initial pH, medium volume in a 250 mL
flask and shaking speed, respectively, resulting in an increase of PLA2 activity from (1.87±0.12) U to (5.35±0.27) U.

Key words: Kluyveromyces lactis, phospholipase A2, fermentation

CLC Number: