Partial DNA Sequence Analysis of 16S rRNA Gene in Puffer Fish

doi:10.7506/spkx1002-6630-201521027

Abstract

Abstract:

Partial fragments of the 16S rRNA gene of puffer fish from 13 species belonging to 3 genus collected from Fujian
province and 9 samples from unknown species were amplified by polymerase chain reaction (PCR) using the universal
16S rRNA gene primers. The PCR products were sequenced, and the length of the obtained DNA fragments were 611–614
bp in all samples. The homology of DNA sequences among samples was analyzed with the DNAMAN V6 software, and
the phylogenetic tree among samples was established. Thirteen samples were divided into 4 groups, with a homology of
87% among groups and 94%–100% between any two samples within the same group. Nine samples of unknown puffer fish
species were classed into 3 groups which belonged to the Gastrophysus or Takifugu genus. The results obtained in this study
can provide references for the application of 16S rRNA gene sequencing and homological analysis to identify puffer fish at
genus and species levels.

Key words: puffer fish, polymerase chain reaction, 16S rRNA gene, DNA sequencing, species identification

CLC Number:

S917.4
Q78

CHEN Wenbing, WENG Guozhu, CHEN Rongbin, MIAO Tingyu, SHAO Biying, JIANG Shuxun, PENG Juan. Partial DNA Sequence Analysis of 16S rRNA Gene in Puffer Fish[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201521027.

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Partial DNA Sequence Analysis of 16S rRNA Gene in Puffer Fish

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