Abstract:

In the present study, the effects of different incubation conditions on the production of zearalenone (ZEN) by
Fusarium graminearum were explored. Culture medium, incubation time, incubation temperature, shaking speed and
light irradiation time were selected as important parameters affecting ZEN production. Single factor method was used in
combination with orthogonal array design and response surface methodology to optimize the medium composition and
culture conditions employing statistical analysis, respectively. The results showed that the optimal culture medium for
Fusarium graminearum was comprised of 60 g/L C6H12O6, 1.5 g/L KNO?, 1.0 g/L yeast extract paste, 20 g/L peptone,
6.0 g/L NaNO3, 0.5 g/L MgSO4, 1.0 g/L K2HPO4·3H2O, 0.5 g/L KCl, and 0.025 g/L Fe2(SO4)3 in 1.0 L of ultrapure water.
The concentration of ZEN was 249.80 μg/L when the cultivation was carried out for 20 days with a shaking speed of
92 r/min, 22.9 ℃ under light irradiation for 10 h a day.

Key words: Fusarium graminearum, zearalenone, enzyme-linked immunosorbent assay, mycotoxin