Abstract:

In this study, response surface methodology based on Box-Behnken design was used to optimize the culture
conditions for the production of the bioactive metabolite antroquinonol in solid-state fermentation of Antrodia camphorata.
The optimal culture conditions were determined as inoculum amount of 296.80 mg/kg, Triton X-100 concentration of
1.10 mL/kg and coenzyme Q0 concentration of 0.23 g/kg. Under these conditions, the maximum predicted yield of
antroquinonol was 865.85 mg/kg, which was in good agreement with the experimental value of 865.32 mg/kg. Therefore, the
established model could be used for predicting the culture conditions of Antrodia camphorate for antroquinonol production.
After optimization, the yield of antroquinonol was increased 232.09% when compared with the control (260.57 mg/kg).

Key words: Antrodia camphorate, solid-state fermentation, antroquinonol, response surface methodology