Abstract:

Objective: To investigate the protective effect of purified polyphenols from leaves of Punica granatum L. (PPPL)
on kidney, heart, spleen and thymus in a mouse model of acute alcohol-induced hepatic injury. Methods: Sixty-six male mice
were randomly divided into blank control group, alcoholic injury model group, positive control group (administered with
150 mg/(kg·d) bifendate by gavage) and PPPL low, medium, and high dose groups (100, 200 and 400 mg/(kg·d)). All mice
were administered for 30 days. On the next day, the mice from all groups except the blank control group were administered with
50% alcohol (12 mL/kg), and then all the mice were subjected to dislocation death to assay the activities of SOD, CAT and GSH-Px
and the contents of MDA and GSH in kidney, heart, spleen and thymus. Results: Compared with the alcoholic injury model
group, the activities of SOD, CAT and GSH-Px and the contents of GSH in kidney, heart, spleen and thymus revealed an obvious
increase in the PPPL treatment groups while the contents of MDA were obviously decreased. Conclusion: PPPL has pronounced
antioxidant effect on kidney, heart and immune organs in mice with alcoholic hepatic injury.

Key words: purified polyphenols from leaves of Punica granatum L. (PPPL), alcohol liver injury, antioxidantion, heart, kidney, immune organs