Abstract:

The ultrasound-assisted Soxhlet extraction of sea buckthorn oil from dry pulp was optimized by orthogonal array
design, and the fatty acid composition and contents of sea buckthorn pulp oil were determined by gas chromatography (GC)
coupled with time-of-flight mass spectrometry (TOF-MS). Using combination of single-factor and orthogonal experiments,
the optimal conditions for sea buckthorn fruit oil extraction were as follows: solid/solvent ratio, 1:70 (g/mL); ultrasonicassisted
extraction time, 30 min; extraction temperature, 55 ℃; and Soxhlet reflux extraction time, 6 h. Under the optimized
conditions, the yield of sea buckthorn oil from dry pulp was 28.14%. Twenty-four fatty acids were detected from the oil,
and the main fatty acids palmitoleic and palmitic acid were 35.56% and 59.37%, respectively, which are widely used in
chemical, health products and cosmetics. Therefore the sea buckthorn fruit oil extracted by the ultrasound-assisted Soxhlet
extraction method can provide a high-quality, abundant raw material for medicine and industrial production.

Key words: sea buckthorn pulp oil, ultrasonic, Soxhlet extraction, gas chromatography coupled with time-of-flight mass spectrometry (GC-TOF-MS), fatty acids