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Simultaneous Determination of Monosaccharides, Disaccharides, and Fructooligosaccharides in Inulin Hydrolysates by High Performance Anion Exchange Chromatography

XU Yanbing1, ZHENG Zhaojuan2,3, XU Ying1, SUN Xiucheng1, XU Qianqian2, OUYANG Jia2,3,*   

  1. 1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
    2. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;
    3. Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing 210037, China
  • Online:2016-01-25 Published:2016-01-15
  • Contact: OUYANG Jia

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Abstract:

An analytical method for the simultaneous determination of glucose, fructose, sucrose, kestose, nystose, and
fructofuranosylnystose in fructooligosaccharide products was developed by using high performance anion exchange
chromatography coupled with pulsed ampere detection (HPAEC-PAD). The analysis was performed on a CarboPacTM PA10
column（250 mm × 2 mm）by gradient elution using NaOH-NaAc as the mobile phase. The column temperature was set at 30 ℃,
and the flow rate was 0.3 mL/min. The results showed that the calibration curves developed for glucose, fructose, sucrose, kestose,
nystose and fructofuranosylnystose had a good linear relationship within the concentration range of 0.1–10 mg/L (r2 > 0.996 1).
The relative standard deviation for six repetitive determinations of each sugar was between 2.69% and 7.21%. This method is
convenient, simple, and sensitive for the detection of fructooligosaccharide products from the enzymatic hydrolysis of inulin.

Key words: high performance anion exchange chromatography, inulin, fructooligosaccharides

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