Abstract:

A novel disposable microfludic chip was developed for rapid detection of pesticide residues in agricultural
products, which integrated sampling, enzyme inhibition reaction, color development and detection chamber. By coupling
with a laboratory-prepared colorimetric detection device, the chip could provide a simple, easy to use, low-cost and sensitive
approach for rapid detection of organophosphate and carbamate pesticides on site. Due to pre-storage of biochemical
reagents in the chip, the detection could be achieved within 7 min by just one injection. The limits of detection (LOD) of
the method were 0.02 mg/L for carbofuran and 0.6 mg/L for dimethoate, the recoveries of carbofuran from spiked samples
were in the range of 95.0% to 103.3%. Furthermore, this microfludic chip had a long storage life of up to 30 days. The
microfluidic chip coupled with laboratory-prepared detection device is expected to achieve an automatic detection process,
and is particularly suitable for on-site, rapid, and high-throughput screening of pesticide residues by non-professionals.

Key words: microfludic chip, pesticide residues, rapid detection, low cost