Abstract:

An analytical method for biogenic amines in Chinese rice wine by reversed phase high-performance liquid
chromatography (RP-HPLC-HPLC) was developed. The biogenic amines in Chinese yellow wine were extracted with
trichloroacetic acid (TCA), and then pre-column derivatized with dansyl chloride for quantification using 1,7-diamino
heptane as the quantitative internal standard. A C18 chromatographic column (4.5 mm × 250 mm, 3.8 μm) was used
for separation. The mobile phase was composed of a mixture of acetonitrile and water at a flow rate of 1 mL/min, and
the ultraviolet (UV) detection wavelength was 254 nm. The results showed that nine biogenic amines (pyridoxamine
dihydrochloride, tryptamine, putrescine, cadaverine, beta phenethylamine, histamine, tyramine, spermine and spermine)
were separated completely within 40 minutes. In the concentration range of 1–50 mg/L, each biogenic amine presented good
linear correlation (R2 > 0.999). After the addition of a mixed standard solution of biogenic amines to Chinese yellow wine,
the recovery rates were 79.54%–89.55%, with relative standard deviations (RSDs) ranging from 3.14% to 6.35%, and the
limit of detection for each component was 0.002–0.009 mg/L. Therefore, The HPLC method with pre-column derivatization
and UV detection could be used for the determination of biogenic amines in Chinese yellow wine.

Key words: biogenic amine, Chinese yellow wine, high performance liquid chromatography (HPLC), gradient elution