Abstract:

Purpose: To optimize the extraction conditions for anthocyanins from Padus racemosa fruits by cellulose
hydrolysis, and to detect the protective effect of the extracted anthocyanins on H2O2-induced damage in Ins-1 cells. Methods:
The optimization was performed by single factor and orthogonal array experiments. The protective effect of Padus racemosa
anthocyanins on Ins-1 cells were examined by morphological observation, MTT assay and DCFH-DA fluorescent probe
method. Results: The optimal extraction conditions were achieved by carrying out cellulose hydrolysis at 60 ℃, pH 3.5 and
an enzyme dosage of 9 mg/g with a solid/liquid ratio of 1:35 (g/mL). Under these conditions, the yield of Padus racemosa
anthocyanins was (0.956 ± 0.027) mg/g. Padus racemosa anthocyanins protected Ins-1 cells from damage induced by H2O2
as indicated by morphological observation and MTT assay, and strongly scavenged reactive oxygen species in the DCFHDA
fluorescent assay. Conclusions: Enzymatic hydrolysis is an effective method for extracting anthocyanins from Padus
racemosa, and the extracted anthocyanins strongly protect against H2O2-induced damage in Ins-1 cells.

Key words: anthocyanin, enzyme, oxidative damage, protection, rat insulinoma (Ins-1) cell