Isolation and Identification of Dominant Spoilage Bacteria from Litopenaeus vannamei and Detection of Quorum Sensing Signaling Molecules

doi:10.7506/spkx1002-6630-201608029

Abstract

Abstract:

This study was aimed at isolating, identifying the dominant spoilage bacteria from Litopenaeus vannamei using
an automatic biochemical identification system and 16S rDNA sequence, detecting the quorum sensing signaling molecules
acyl homoserine lactones (AHLs), autoinducer-2 (AI-2) and exploring the dynamic formation regularities with indicator
bacteria Agrobacterium tumefaciens JZA-1 and Vibrio harveyi BB170, respectively. Ten strains were selected and identified,
including Oceanisphaera profunda, Stapylococcus haemolyticus, Shewanella putrefaciens, Brevibacterium sp., Bacillus
siamensis, Exiguobacterium aaestuarii and four strains of the Proteus genus. All the Gram-negative spoilage organisms isolated
secreted signaling molecule AHLs while Proteus sp., Shewanella putrefaciens, Brevibacterium sp. and Bacillus siamensis secreted
signaling molecule AI-2. The AHLs activity of dominant spoilage organism was positively correlated with cell density. The AI-2
production was accumulated within the logarithmic phase and decreased rapidly from 24 h onwards.

Key words: Litopenaeus vannamei, dominant spoilage organism, quorurm sensing, signaling molecule

CLC Number:

TS254.1

LI Can, ZHANG Yuchen, SUN Lijun, WANG Yaling, LIANG Meijing, HU Hanqiao, XU Defeng, LIU Ying, YE Riying. Isolation and Identification of Dominant Spoilage Bacteria from Litopenaeus vannamei and Detection of Quorum Sensing Signaling Molecules[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201608029.

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[2]	WANG Lijuan, HU Zhihe, WANG Xingxuan, LI Zhiqing, LUO Mengjuan, PAN Zimeng, YANG Yujuan, LIU Lifeng, LIU Li. WANG Lijuan, HU Zhihe, WANG Xingxuan, LI Zhiqing, LUO Mengjuan, PAN Zimeng, YANG Yujuan, LIU Lifeng, LIU Li [J]. FOOD SCIENCE, 2019, 40(20): 299-304.
[3]	DUAN Weiwen, QUAN Qinguo, GAO Jing, MAO Weijie, HAO Jiming, LIU Shucheng, JI Hongwu. Effect of Low-Voltage Electrostatic Field Combined with Modified Atmosphere Packaging on the Quality of Litopenaeus vannamei during Controlled Freezing-Point Storage [J]. FOOD SCIENCE, 2019, 40(13): 252-259.
[4]	SUN Jiangping, ZHAO Li, YU Wenying, XIE Jing, PAN Yingjie, ZHAO Yong. Effect of Acidic Electrolyzed Water on Polyphenoloxidase Activity from Litopenaeus vannamei [J]. FOOD SCIENCE, 2018, 39(9): 7-12.
[5]	LIU Shucheng, GUO Minghui, LIU Yuan, JI Hongwu, GAO Jing, MAO Weijie, DENG Chujin, HAO Jiming, LUO Shuai, DONG Andi. Change in Intermolecular Force of Myosin from Litopenaeus vannamei Treated by Dense Phase Carbon Dioxide [J]. FOOD SCIENCE, 2018, 39(7): 153-159.
[6]	DENG Xiaoying, ZHANG Bin, TANG He, HAO Guijuan, ZHANG Yangyang. Analysis of Microbial Diversity in Shrimp (Litopenaeus vannamei) by MiSeq High-Throughput Sequencing [J]. FOOD SCIENCE, 2018, 39(24): 149-155.
[7]	ZHANG Yangyang, ZHANG Bin, HAO Guijuan, DENG Xiaoying, TANG He. Effect of Sugar Alcohols on Water Retention in Frozen Shrimp (Litopenaeus vannamei) [J]. FOOD SCIENCE, 2018, 39(23): 170-175.
[8]	JIA Ying, HU Zhihe. Changes in Allergenicity and Sensory Quality of Peeled Shrimp Caused by Ultra-High Pressure [J]. FOOD SCIENCE, 2017, 38(9): 14-20.
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[10]	WU Haixiao, ZHANG Bin, SHI Zhourong, FANG Chuandong, ZOU Junjie, LI Haibo. Cryoprotective Effects of Carrageenan Oligosaccharides on Pacific White Shrimp (Litopenaeus vannamei) during Frozen Storage [J]. FOOD SCIENCE, 2017, 38(7): 260-265.
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Isolation and Identification of Dominant Spoilage Bacteria from Litopenaeus vannamei and Detection of Quorum Sensing Signaling Molecules

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