Isolation, Purification and Antioxidant Activity of Polysaccharides from Zizyphus jujube cv. Dongzao

doi:10.7506/spkx1002-6630-201613016

Abstract

Abstract:

Objective: To study the monosaccharide composition, structure and antioxidant activity of polysaccharide
fractions isolated and purified from Zizyphus jujube cv. Dongzao. Methods: The extraction of polysaccharides was performed
by water extraction and subsequent ethanol precipitation, and after removal of proteins and pigments, the polysaccharides
were purified by successive DEAE-52 cellulose column and Sephadex G-100 gel column chromatography. The purity
and molecular weights of the purified polysaccharides were determined by Sephadex G-100 column chromatography.
Preliminary structural characterization was carried out by ultraviolet-visible (UV-VIS) spectroscopy, infrared (IR)
spectroscopy and gas chromatography (GC). The antioxidant activity of the crude and purified polysaccharides was evaluated
using the phenanthroline method and 1, 1-diphenyl-2-picrylhydroxyl (DPPH) radical scavenging activity assay. Results:
Two polysaccharide fractions (DPA and DPB) were purified to homogeneity from the crude polysaccharides as identified by
Sephadex G-100. The molecular weight of DPA and DPB was 1.04 × 104 and 3.02 × 105 D, respectively. DPA and DPB did
not contain protein or nucleic acid, but contained the pyranose ring skeleton glucoside. The monosaccharide composition
of DPA consisted of arabinose, mannose, glucose and galactose with a molar ratio of 6.66:1.00:6.75:2.09, whereas that of
DPB consisted of rhamnose, arabinose, mannose, glucose and galactose with a molar ratio of 4.33:10.90:1.00:3.25:4.78.
The crude polysaccharides, DPA and DPB all had antioxidant activity in a concentration dependent manner. The scavenging
percentages of DPA and DPB at 8 mg/mL for hydroxyl radical were 28.52% and 78.79% respectively, which were 9.97%
and 24.54% at 0.4 mg/mL, respectively. Conclusion: Both DPA and DPB had antioxidant activity.

Key words: Zizyphus jujube cv. Dongzao, polysaccharide, isolation and purification, structure identification, antioxidant activity

CLC Number:

TS201.2

PAN Ying, XU Jingwei. Isolation, Purification and Antioxidant Activity of Polysaccharides from Zizyphus jujube cv. Dongzao[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613016.

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