Effect of Hydrolyzed Wheat Protein Peptide on Ethanol-Induced Acute Gastric Mucosal Damage in Rats

doi:10.7506/spkx1002-6630-201613032

Abstract

Abstract:

Objective: The present work was aimed to examine the protective effect of hydrolyzed wheat protein peptide
(HWP) in the model rats with acute gastric mucosal damage induced by ethanol. Methods: Hydrolyzed wheat protein
peptide (167, 333 or 667 mg/(kg·d) (in body mass), cimetidine (65 mg/(kg·d)), or distilled water were administered daily by
gavage for 30 days before the rats were treated with anhydrous ethanol (1 mL) and then euthanized after 1 h. Histological
observation was performed to evaluate the antioxidant and anti-inflammatory activity of HWP as well as its impact on serum
physiological and biochemical parameters and the expression of caspase-3 and epidermal growth factor receptor (EGFR).
Results: Histological observation showed that ethanol-induced gastric mucosal damage was attenuated by hydrolyzed
wheat protein peptide pretreatment. Hydrolyzed wheat protein peptide remarkably increased the superoxide dismutase
(SOD) activity, epidermal growth factor (EGF) and prostaglandin E-2 (PGE2) levels in serum. Furthermore, it dramatically
decreased malondialdehyde (MDA), platelet-activating factor (PAF) and interleukin-8 (IL-8) levels in serum. The expression
level of caspase-3 in stomach was significantly decreased in hydrolyzed wheat protein peptide-treated rats when compared
with the model group; however, the expression level of EGFR was markedly increased. Conclusion: Ethanol-induced gastric
mucosal damage in rats can be protected against by hydrolyzed wheat protein peptide. The potential mechanism may be
associated with the enhancement of antioxidant, anti-inflammatory and anti-apoptotic functions.

Key words: hydrolyzed wheat protein peptide, gastric mucosal damage, ethanol

CLC Number:

R151

YANG Xian, WANG Yanyan, WANG Feng, XIA Hui, PAN Xingchang, ZHU Hangju, GU Ruizeng, MA Yongqing, TANG Huali, WANG Shaokang, SUN Guiju. Effect of Hydrolyzed Wheat Protein Peptide on Ethanol-Induced Acute Gastric Mucosal Damage in Rats[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613032.

References

[1] 陈建章, 陈文, 喻菁, 等. 半四左方对胃溃疡大鼠表皮细胞生长因

子和转化生长因子的影响[J]. 时珍国医国药, 2014(2): 313-314.

DOI:10.3969/j.issn.1008-0805.2014.02.024.

[2] 何绍珍, 任建林. 乙醇对胃黏膜作用机制的研究进展[J]. 世界华

人消化杂志, 2 0 0 5 , 1 3 ( 2 1 ) : 2591-2596. DOI:10.3969/

j.issn.1009-3079.2005.21.016.

[3] YIN H, CAI H Z, WANG S K, et al. Wheat peptides reduce oxidative

stress and inhibit NO production through modulating mu-opioid

receptor in a rat NSAID-induced stomach damage model[J]. Chinese

Journal of Natural Medicines, 2015, 13(1): 22-29. DOI:10.1016/

S1875-5364(15)60003-6.

[4] JEONG H J, JEONG J B, KIM D S, et al. The cancer preventive

peptide lunasin from wheat inhibits core histone acetylation[J]. Cancer

Letters, 2007, 255(1): 42-48. DOI:10.1016/j.canlet.2007.03.022.

[5] MOTOI H, KODAMA T. Isolation and characterization of angiotensin

I-converting enzyme inhibitory peptides from wheat gliadin

hydrolysate[J]. Nahrung-Food, 2003, 47(5): 354-358. DOI:10.1002/

food.200390081.

[6] NIU L Y, JIANG S T, PAN L J. Preparation and evaluation of

antioxidant activities of peptides obtained from defatted wheat germ

by fermentation[J]. Journal of Food Science and Technology-Mysore,

2013, 50(1): 53-61. DOI:10.1007/s13197-011-0318-z.

[7] DADZIE R G, MA H L, ABANO E E, et al. Optimization of process

conditions for production of angiotensin I-converting enzyme (ACE)

inhibitory peptides from vital wheat gluten using response surface

methodology[J]. Food Science and Biotechnology, 2013, 22(6): 1531-1537.

DOI:10.1007/s10068-013-0248-9.

[8] 国家食品药品监督管理局. 关于印发抗氧化功能评价方法等9个保

健功能评价方法的通知[EB/OL]. (2012-04-23)[2016-05-23]. http://

www.sda.gov.cn/WS01/CL0847/71257.html.

[9] AMIRSHAHROKHI K, KHALILI A R. The effect of thalidomide

on ethanol-induced gastric mucosal damage in mice: involvement

of inflammatory cytokines and nitric oxide[J]. Chemico-Biological

Interactions, 2015, 225: 63-69. DOI:10.1016/j.cbi.2014.11.019.

[10] 问慧娟, 李玉巧, 崔玉英, 等. 白藜芦醇对大鼠应激性胃溃疡的保

护作用[J]. 中国医院药学杂志, 2014, 34(1): 15-18. DOI:10.13286/

j.cnki.chinhosppharmacyj.2014.01.05.

[11] CHEN H M, LIAO H J, LIU Y H, et al. Protective effects of

pogostone from Pogostemonis herba against ethanol-induced gastric

ulcer in rats[J]. Fitoterapia, 2015, 100: 110-117. DOI:10.1016/

j.fitote.2014.11.017.

[12] NAKAMURA T, SAKAGUCHI T, UNNO N, et al. Relationship

between the platelet activating factor acetylhydrolase gene and

intractability of ulcerative colitis[J]. Diseases of the Colon & Rectum,

2002, 45(3): 389-393. DOI:10.1007/s10350-004-6188-y.

[13] 朱焕金. 健脾益气方药调控血小板活化因子对急性胃黏膜损伤修

复作用的研究[D]. 广州: 广州中医药大学, 2009: 10-12.

[14] ARAKAWA T, HIGUCHI K, FUJIWARA Y, et al. 15th Anniversary

of rebamipide: looking ahead to the new mechanisms and new

applications[J]. Digestive Diseases and Sciences, 2005, 50(Suppl 1):

3-11. DOI:10.1007/s10620-005-2800-9.

[15] 阮研硕, 赵江燕, 李艳梅, 等. 岩藻多糖对急性酒精性胃黏膜损伤

的保护作用[J]. 中国食品学报, 2015, 15(1): 19-24. DOI:10.16429/

j.1009-7848.2015.01.004.

[16] 曲宝戈, 潘锦敦, 王中东, 等. 长期饮酒胃黏膜病理改变与PGE2

和EGF的关系[J]. 胃肠病学和肝病学杂志, 2011, 20(9): 801-803.

DOI:10.3969/j.issn.1006-5709.2011.09.006.

[17] 张雅博, 阮研硕, 赵晓红, 等. 乙醇性胃黏膜损伤及其保护作用的研

究进展[J]. 食品安全质量检测学报, 2013(4): 1239-1245.

[18] 黄进波, 萧钦, 周佳佳, 等. 竹叶黄酮对乙醇诱导小鼠急性胃黏膜损

伤的保护作用[J]. 现代药物与临床, 2015(7): 779-783. DOI:10.7501/

j.issn.1674-5515.2015.07.006.

[19] YANAKA A, SUZUKI H, SHIBAHARA T, et al. EGF promotes

gastric mucosal restitution by activating Na+/H+ exchange of

epithelial cells[J]. American Journal of Physiology-Gastrointestinal

and Liver Physiology, 2002, 282(5): G866-G876. DOI:10.1152/

ajpgi.00150.2001.

[20] 韦维, 林寿宁, 黄贵华, 等. 安胃汤对胃溃疡大鼠溃疡修复及表

皮生长因子的影响[J]. 湖南中医药大学学报, 2008, 28(6): 38-40.

DOI:10.3969/j.issn.1674-070X.2008.06.011.

[21] 刘晓玲, 王汝俊, 张千桥. 补中益气汤对脾虚大鼠胃黏膜EGFR蛋

白表达的影响[J]. 中药药理与临床, 2012(5): 12-14. DOI:10.13412/

j.cnki.zyyl.2012.05.009.

[22] 文国容, 徐靖宇, 刘雪梅, 等. 胃乐散对大鼠胃溃疡黏膜EGF、

EGFR和TGFβ1的影响及意义[J]. 时珍国医国药, 2012, 23(1): 3-4.

DOI:10.3969/j.issn.1008-0805.2012.01.002.

[23] YANG Z B, YAN J, ZOU X P, et al. Enhanced expression of

epidermal growth factor receptor gene in gastric mucosal cells by the

serum derived from rats treated with electroacupuncture at stomach

meridian acupoints[J]. World Journal of Gastroenterology, 2006,

12(34): 5557-5561. DOI:10.3748/wjg.v12.i34.5557.

[24] 于海, 柴剑波, 赵伟国, 等. 大黄黄连泻心汤、理中丸对消炎痛型胃

溃疡寒热证模型大鼠血清IL-8和TNF-α含量的影响[J]. 时珍国医国

药, 2012(2): 382-383. DOI:10.3969/j.issn.1008-0805.2012.02.058.

[25] 陈建章, 陈文, 王小平, 等. 半四左方对乙酸型胃溃疡的愈合作

用及IL-8和IL-1β的影响[J]. 时珍国医国药, 2015(4): 863-864.

DOI:10.3969/j.issn.1008-0805.2015.04.036.

[26] YAKABI K, MIMURA H, IWABUCHI H, et al. Neutrophilderived

hydroxyl radicals mediate interleukin-8-induced increases in

tetragastrin-stimulated acid secretion in rats[J]. Digestive Diseases and

Sciences, 2003, 48(6): 1081-1087. DOI:10.1023/A:1023708511116.

[27] VISCONTI R, D’ADAMIO L. Functional cloning of genes regulating

apoptosis in neuronal cells[J]. Methods in Molecular Biology, 2007,

399: 125-131. DOI:10.1007/978-1-59745-504-6_9.

[28] SLOMIANY B L, SLOMIANY A. Ghrelin protection against

lipopolysaccharide-induced gastric mucosal cell apoptosis involves

constitutive nitric oxide synthase-mediated caspase-3 s-nitrosylation[J].

Mediators of Inflammation, 2010: 1-7. DOI:10.1155/2010/280464.

[29] 白丽君. 心身1号对慢性应激致胃黏膜损伤大鼠c-fos, caspase-3,

c-myc的影响[D]. 天津: 天津医科大学, 2009: 23-27.

[30] LOU L X, GENG B, YU F, et al. Endoplasmic reticulum stress

response is involved in the pathogenesis of stress induced gastric lesions

in rats[J]. Life Sciences, 2006, 79(19): 1856-1864. DOI:10.1016/

j.lfs.2006.06.022.