Determination of Aildenafil Illegally Added in Health Wine by High Performance Liquid Chromatography-Tandem Mass Spectrometry

doi:10.7506/spkx1002-6630-201614036

Abstract

Abstract:

Objective: To develop a detection method for aildenafil adulterated in health wine using high performance liquid
chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods: The chromatography was performed using an
Agilent RRDH Eclipse plus C18 column (2.1 mm × 100 mm, 1.8 μm), with a mobile phase consisting of acetonitrile-0.1%
formic acid (phase A) and 0.1% acetic acid -20 mmol/L ammonium acetate (phase B) by gradient elution. Qualitative
analysis of aildenafil was conducted under full-scan model, and quantitative analysis in the multiple reaction monitoring
(MRM) model. Results: The method was linear with a correlation coefficient (r2) > 0.99, and its precision, expressed as
RSD, was < 3%. The recoveries of aildenafil at different spiked concentrations ranged from 97.31% to 100.66%. The limit
of detection (LOD) was 0.6 ng/mL and the limit of quantitation (LOQ) was 1.6 ng/mL. Conclusion: This method is fast and
accurate with a high sensitivity and a high degree of separation. It can be used for the screening and detection of aildenafil
illegally added in health wine.

Key words: aildenafil, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), health wine, detection method, illegally added

CLC Number:

TS207.3

ZHANG Liyuan, YAN Linqi, ZHOU Minghao, WU Yuanyang. Determination of Aildenafil Illegally Added in Health Wine by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614036.

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