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Differences of Semicarbazide Content in Animal-Derived Foods Disinfected by Peroxyacetic Acid Disinfectant

YE Gang1, LIANG Tongwen1, HUAI Wenbei2, XIE Dongdong1, SHEN Jincan1,*, ZHANG Yi1, KANG Haining1   

  1. 1. Shenzhen Key Laboratory of Detection Technology R&D on Food Safety, Food Inspection and Quarantine Center of Shenzhen
    Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China; 2. Traditional Chinese Medicine College,
    Guangdong Pharmaceutical University, Guangzhou 528458, China
  • Online:2016-09-15 Published:2016-09-22

Abstract:

Peroxyacetic acid is widely used in animal-derived foods disinfection since it is an effective and broad spectrum
disinfectant; however, our experiments have found that semicarbazide could be detected in samples treated by peroxyacetic
acid disinfectant. To study different contents of semicarbazide after treatment with peroxyacetic acid under different
conditions, the effects of peroxyacetic acid concentration, temperature, treatment time and different animal-derived foods
samples on the generation of semicarbazide were investigated. After samples were treated with peroxyacetic acid solution,
excessive Na2S2O3 was added to remove the unreacted peroxyacetic acid for the derivatization of semicarbazide into
2-nitrobenzaldehyde under acidic conditions. The preparation was extracted with ethyl acetate, and then the extract was
detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and quantified by isotope internal standard
method. The results showed that peroxyacetic acid concentration, temperature and treatment time had a significant positive
correlation with the yield of semicarbazide, respectively.

Key words: peroxyacetic acid, disinfection, animal-derived foods, semicarbazide

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