Abstract:

The objective of this research was to investigate the antioxidant role in endothelial cells. The influence of
angiotensin II (Ang II) on the expression of xanthine oxidase-1 (XO-1) and heme oxygenase-1 (HO-1) in human umbilical
vein endothelial cells (HUVECs), and the changes in reactive oxygen species (ROS), superoxide dismutase (SOD), XO-1,
and HO-1 caused by two malvidin glycosides malvidin-3-glucoside (Mv-3-glc) and malvidin-3-galactoside (Mv-3-gal) were
investigated. The levels of XO-1, HO-1, and SOD were evaluated by enzyme linked inmunosorbent assay (ELISA) kits.
ROS level was detected under a fluorescence microplate. Compared with the control group, the expression of XO-1 protein
was significantly increased and the expression of HO-1 protein was significantly decreased in the 10-2 μmol/L Ang II-treated
group. Different concentrations of Mv-3-glc and Mv-3-gal as well as their mixture greatly decreased the levels of ROS
and XO-1, but increased the levels of SOD and HO-1. Glycosides improved the antioxidant capacity of malvidin to a great
extent. The antioxidant activity of Mv-3-glc was more pronounced than that of Mv-3-gal. Variation in malvidin glycosides
had a significant impact on Ang II-induced oxidative stress to different extents. Therefore, malvidin glycosides can protect
cells from oxidative deterioration and thus used to prevent diseases associated with oxidative stress.

Key words: malvidin-3-glucoside, malvidin-3-galactoside, endothelial cells, angiotensin II (Ang II), reactive oxygenspecies (ROS), oxidative stress