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Purification and Antitumor Activity in vitro of Polysaccharides from Apostichopus japonicus Spawn

LIU Xin1, LIU Jingxi1, ZHANG Jian1,2,*, WANG Ting3, WANG Gongming1, JING Yuexin1, ZHAO Yunping1   

  1. 1. Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;
    2. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai 264006, China;
    3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2016-12-15 Published:2016-12-21

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Abstract: Purpose: To investigate the structural characteristics and antitumor activity in vitro of polysaccharides from
Apostichopus japonicus spawn. Methods: The polysaccharides from Apostichopus japonicus spawn (SPS) were isolated by
protease hydrolysis and alcohol precipitation, and then purified by chromatography. Their physicochemical properties were
analyzed. Their inhibitory effect on the growth of HeLa and HGC-27 cells was evaluated using methyl thiazolyl tetrazolium
assay. Results: SPS-1, SPS-2 and SPS-3 were purified from SPS. The proliferation of two tumor cells was inhibited by
intervention of each of the three polysaccharides. SPS-1 showed the strongest antitumor activity, with a percentage inhibition
of 98.04% and 99.4% for HeLa and HGC-27 cells, respectively. Conclusion: Three different polysaccharides with the ability
to inhibit tumor cell proliferation have been obtained from A. japonicus spawn.

Key words: Apostichopus japonicus spawn, polysaccharide, purification, antitumor activity

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