Determination of Trace Selenium in Enzymatic Hydrolysates of Rice by Direct Injection-Inductively Coupled
Plasma-Mass Spectrometry

doi:10.7506/spkx1002-6630-201710028

FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (10): 168-172.doi: 10.7506/spkx1002-6630-201710028

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Determination of Trace Selenium in Enzymatic Hydrolysates of Rice by Direct Injection-Inductively Coupled Plasma-Mass Spectrometry

ZHAO Ermin , FANG Yong , WANG Mingyang , LI Peng , HU Qiuhui , QIU Weifen

1. Key Laboratory of Grains and Oils Quality Control and Processing of Jiangsu Province, Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China; 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Online:2017-05-25 Published:2017-05-23

Abstract

Abstract: A new method was developed for the determination of trace selenium (Se) in enzymatic hydrolysates of rice by direct injection-inductively coupled plasma-mass spectrometry (ICP-MS), and applied to evaluate the Se bioaccessibility during in vitro gastrointestinal digestion. After filtration and centrifugation, the prepared samples were determined by direct injection-ICP-MS. The proposed method showed a good linearity in the concentration range of 0–160 μg/L with correlation coefficient above 0.999 5 and a limit of detection (LOD) of 0.009 μg/L. The recoveries of spiked samples were between 95.3% and 102.8%, and the relative standard deviations (RSDs) were in the range of 0.87% to 4.48%. Furthermore, the direct injection-ICP-MS method was successfully applied to determine trace Se in in vitro gastrointestinal digestion products of cooked rice with short analysis time and environmental friendliness. In conclusion, this method can be used in nutritional evaluation in vitro of Se-enriched grain materials.

Key words: direct injection, inductively coupled plasma-mass spectrometry (ICP-MS), selenium, in vitro gastrointestinal digestion

CLC Number:

S132

ZHAO Ermin , FANG Yong , WANG Mingyang , LI Peng , HU Qiuhui , QIU Weifen. Determination of Trace Selenium in Enzymatic Hydrolysates of Rice by Direct Injection-Inductively Coupled Plasma-Mass Spectrometry[J]. FOOD SCIENCE, 2017, 38(10): 168-172.

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Determination of Trace Selenium in Enzymatic Hydrolysates of Rice by Direct Injection-Inductively Coupled Plasma-Mass Spectrometry

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