FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (13): 182-189.doi: 10.7506/spkx1002-6630-201713030

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect of Urolithin A on Macrophage Polarization and the Formation of Macrophage-Derived Foam Cells

HAN Qi’an, LIU Hongyan, YAN Chunhong, SHI Chao, XIA Xiaodong   

  1. 1. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China; 2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University,Beijing 100083, China; 3. Dingxing Hospital, Dingxing 072650, China; 4. School of Life Science and Technology,Xi’an Jiaotong University, Xi’an 710049, China
  • Online:2017-07-15 Published:2017-07-11

Abstract: This research was designed to investigate the potential anti-atherogenic effect of urolithin A on macrophage polarization and the formation of macrophage derived-foam cells as well as the underlying molecular mechanisms. It was found that urolithin A could mediate the mRNA expression of different marker genes in M1-type and natural macrophages, indicating its ability to inhibit the polarization of macrophage cell line (RAW264.7) into pro-inflammatory M1-type macrophage and to promote the polarization of macrophage cells into M2 type. The results of oil red O staining revealed that urolithin A inhibited the formation of foam cells derived from macrophages. Real time PCR showed that urolithin A also markedly decreased the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase and fatty acid synthase at the transcriptional level. Besides, urolithin A was capable of up-regulating the mRNA expression of the ATP-binding cassette transporters A1 and G1, which was positively associated with macrophage cholesterol efflux. In summary, the findings confirmed the essential role urolithin A plays in macrophage polarization, and further demonstrated the potential molecular mechanisms of urolithin A in preventing foam cell formation and cholesterol synthesis, which are of great significance in investigating and understanding the anti-atherogenic effect of urolithin A in the future.

Key words: urolithin A (Uro-A), macrophage polarization, foam cells, cholesterol, atherosclerosis

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