Optimization of Fermentation Process for the Production of Intracellular Polysaccharide from Chlamydomonas sp. 212 and Its Antimicrobial Activity

doi:10.7506/spkx1002-6630-201718001

Abstract

Abstract: Response surface methodology was applied to optimize the fermentation process of intracellular polysaccharide production from Chlamydomonas sp. 212. Then, the antibacterial activity of intracellular polysaccharide was studied by filter paper disc method. The optimum fermentation medium was found to consist of NaNO3 305.01 mg/L, NaCl 93.66 mg/L, and NaHCO3 2.12 g/L. The bacterium grown in the optimized medium yielded 91.182 3 mg/L of intracellular polysaccharide, which was increased by 160% compared to that obtained using the unoptimized medium. The intracellular polysaccharide from Chlamydomonas sp. 212 had strong antibacterial activity against Salmonella typhimurium and weak antimicrobial activity against Escherichia coli, Staphylococcus albus, Staphylococcus?aureus and Aspergillus niger. This study may provide a theoretical basis for the screening of new natural antimicrobial substances and for the comprehensive utilization of oleaginous microalgae.

Key words: Chlamydomonas sp., intracellular polysaccharide, response surface methodology, fermentation process, antibacterial activity

CLC Number:

Q939.9

SUN Jianrui, FU Dandan, ZHAO Junfeng, WANG Dahong, GU Shaobin. Optimization of Fermentation Process for the Production of Intracellular Polysaccharide from Chlamydomonas sp. 212 and Its Antimicrobial Activity[J]. FOOD SCIENCE, 2017, 38(18): 1-7.

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Optimization of Fermentation Process for the Production of Intracellular Polysaccharide from Chlamydomonas sp. 212 and Its Antimicrobial Activity

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